First Derivative Spectrophotometric Method for the Simultaneous Estimation of Tolperisone and Paracetamol in their Combined Dosage Form

The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective First derivative spectrophotometric zero crossing method for the simultaneous determination of Tolperisone and Paracetamol in combined dosage form. The utility of first derivative data processing program is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The first order derivative absorption at 217.60 nm (zero cross point for Paracetamol) was used for Tolperisone and 223.60 nm (zero cross point for Tolperisone) was used for Paracetamol. The linearity was obtained in the concentration range of 2-20 μg/ml for Tolperisone and 2-12 μg/ml for Paracetamol. The method was successfully applied to synthetic mixture because no interference from excipients was found. The suitability of these methods for the quantitative determination of Tolperisone and Paracetamol was proved by validation. The proposed methods were found to be simple and sensitive for the routine quality control application of Tolperisone and Paracetamol in pharmaceutical dosage form. The results of analysis have been validated statistically and by recovery studies.